The spike seen in the pump out phase is due to the commencement of flow and reagents/material that is outside the optical path initially but is swept through the optical path during flush out. The spike is accepted as normal behavior of the system and does not affect...
HydroCYCLE
How can I identify and fix bubble spike failures in my Hydrocycle data?
First off, let’s see what a relatively good Raw Data Plot looks like. The different phases are the pre-flush phase (dark blue), the ambient baseline reading period (red), the sample reagent pump phase (green), the sample read/reaction phase (purple), and finally the...
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